Open AccessEffect of Myleran On Murine Hemopoiesis
Author(s) -
Delmonte L.,
Williams N.,
Moore M. A. S.
Publication year - 1978
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1978.tb00809.x
Subject(s) - haematopoiesis , spleen , biology , bone marrow , colony forming unit , microbiology and biotechnology , immunology , stem cell , bacteria , genetics
Spleen colony forming cells (CFU‐S) and agar‐gel colony forming cells (CFU‐C) are separate but heterogeneous cell populations, as measured by buoyant density. Myleran (MY) abrogated the major (lighter density) components of the CFU‐S and CFU‐C compartments, thus shifting the surviving CFU‐S and CFU‐C density profiles into the higher density region. the normal spleen colony erythrocytic: granulocytic (E:G) ratio profile showed three density regions with different distributions of erythroid and granulocytic colonies. the preponderantly erythrocytic colony‐generating CFU‐S of the intermediate density regions were eradicated by MY. Comparison of the density distribution of erythrocytic and granulocytic colony‐generating CFU‐S of normal bone marrow showed that the erythrocytic CFU‐S profile paralleled that of total CFU‐S, while most of the granulocytic CFU‐S were contained in the major (and lowest density) peak. MY eradicated the two main (and lowest density) peaks of CFU‐S; surviving CFU‐S occurred preponderantly in a minor (higher density) peak which has a high potential for generating erythrocytic colonies.