Open AccessDissecting the Hematopoietic Microenvironment
Author(s) -
Wolf N. S.
Publication year - 1978
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1978.tb00806.x
Subject(s) - erythropoiesis , haematopoiesis , spleen , bone marrow , stroma , stromal cell , congenic , biology , immunology , stem cell , microbiology and biotechnology , cancer research , medicine , genetics , anemia , gene , immunohistochemistry
Experiments were carried out with the intent of defining the nature of the microenvironmental defect which severely limits erythropoiesis in the spleen of the S1/S1 d mouse. Chimeric spleens, half S1/S1 d and half congenic +/+, supported erythropoiesis in the +/+ region but not in the S1/S1 d region, regardless of which genotype was the irradiated, marrow‐injected host animal. Implants of normal marrow stroma within the spleens of irradiated S1/S1 d mice also supported normal proportions of erythrocytic and granulocytic hematopoiesis. Implants of normal spleen stroma, particularly the capsular portion, into unirradiated S1/S1 d mice stimulated erythropoiesis originating from S1/S1 d stem cells within and in the immediate vicinity of the implant. the evidence suggests a short range, stromal erythropoietic stimulatory factor which is lacking in the S1/S1 d .