Open AccessTHE IN VITRO DIFFERENTIATION OF DENSITY SUB‐POPULATIONS OF COLONY‐FORMING CELLS UNDER THE INFLUENCE OF DIFFERENT TYPES OF COLONY‐STIMULATING FACTOR
Author(s) -
Byrne P.,
Heit W.,
Kubanek B.
Publication year - 1977
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1977.tb00302.x
Subject(s) - biology , in vitro , colony stimulating factor , progenitor cell , bone marrow , agar , colony forming unit , microbiology and biotechnology , differential centrifugation , centrifugation , immunology , haematopoiesis , stem cell , biochemistry , genetics , bacteria
The in vitro proliferation and differentiation of myeloid progenitor cells (CFU‐c) in agar culture from CBA/Ca mouse bone marrow cells was studied. Density sub‐populations of marrow cells were obtained by equilibrium centrifugation in continuous albumin density gradients. The formation of colonies of granulocytes and/or macrophages was studied under the influence of three types of colony‐stimulating factor (CSF) from mouse lung conditioned medium CSF MLCM ), post‐endotoxin mouse serum (CSF ES ) and from human urine (CSF Hu ). The effect of the sulphydryl reagent mercaptoethanol on colony development was also examined. The density distribution of CFU‐c was dependent on the type of CSF. Functional heterogeneity was found among CFU‐c with partial discrimination between progenitor cells forming pure granulocytic colonies and those forming pure macro‐phage colonies. Mercaptoethanol increased colony incidence but had no apparent effect on colony morphology or the density distribution of CFU‐c.