Open AccessPOST‐MITOTIC AGE OF MONONUCLEAR CELLS MIGRATING INTO TA‐3(St) SOLID TUMORS
Author(s) -
Kaizer L.,
Lala P. K.
Publication year - 1977
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1977.tb00296.x
Subject(s) - transplantation , saline , peripheral blood mononuclear cell , mitosis , lymphocyte , monocyte , pathology , andrology , biology , immunology , medicine , endocrinology , in vitro , biochemistry , microbiology and biotechnology
Subcutaneous transplantation of 5 × 10 6 TA‐3(St) mammary carcinoma cells into A/J mice produced rapidly growing tumors that showed a substantial accumulation of lymphocytes, monocytes and macrophages. This must have resulted primarily from an influx of circulating cells, since none of the cell types exhibited any significant local proliferation as indicated by 1 hr 3 H‐TdR uptake. The post mitotic age of the various leukocyte types in circulation of normal and tumor bearing animals, and those appearing within the tumors, was evaluated by a repeated 3 H‐TdR labeling protocol designed to label newly formed leukocytes. Tumor transplantation (or injection of an equivalent volume of saline in control animals) was preceded by thirteen 3 H‐TdR injections (12.5 μCi every 8 hr) and followed by eight more injections at equivalent intervals. Animals were serially sacrificed at 5‐14 days after tumor transplantation or saline injection. Total lymphocyte labeling in the blood during these intervals was higher in tumor bearing mice (approximately 25% between 5 and 12 days) than in the saline injected controls (approximately 20%), indicating that tumor transplantation resulted in an increase in the proportion of young lymphocytes in circulation. More significantly, a much higher labeling (57‐60% at 5‐12 days) was exhibited by lymphocytes isolated from the tumors, indicating selective migration (and/or retention) of newly formed lymphocytes within the tumor. This finding applied to lymphocytes in all size categories. Although blood monocyte labeling in the control and experimental animals was similar (e.g. 83% at day 5 and 52% at day 14), significantly higher labeling (e.g. 93% and 70% at the respective intervals) was exhibited by monocytes isolated from tumors. This suggested a preferential accumulation of young monocytes at the tumor site. An identical macrophage labeling pattern compared to that shown by monocytes within the tumor indicated a rapid monocyte‐macrophage transition. Since these findings in the present strain‐specific solid tumor are similar to those previously obtained in this laboratory in the strain‐nonspecific Ehrlich ascites tumor, the phenomenon of selective localization of newly formed mono‐nuclear leukocytes appears to be a general occurrence in transplanted murine tumors.