Open AccessPROPERTIES OF THE H‐4‐II‐E TUMOR CELL SYSTEM
Author(s) -
Kovacs C. J.,
Evans M. J.,
Hopkins H. A.
Publication year - 1977
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1977.tb00292.x
Subject(s) - cell growth , cell culture , doubling time , cell cycle , biology , cell , plateau (mathematics) , cell division , population , elongation , andrology , microbiology and biotechnology , biochemistry , genetics , materials science , medicine , mathematics , mathematical analysis , environmental health , ultimate tensile strength , metallurgy
The growth and cell proliferation characteristics of the H‐4‐II‐E cell line, giving rise to hepatoma H‐4‐II‐E when inoculated into male ACI rats, were studied in vitro. Following seedling of 2 × 10 3 cells into culture dishes, exponential cell growth occurs in cultures fed both at 24 hr and 48 hr intervals with a population doubling time of 18.4 hr. Plateau phase growth conditions are established on day 7 and day 5 for cultures fed at 24 hr and 48 hr intervals respectively. Both the plateau phase cell density and the maintenance of plateau phase appear dependent on the frequency of feeding. For cultures fed daily, the transition from exponential growth to plateau phase results from both a reduction in the number of proliferating cells (99% v. 35%) as well as an elongation of the cell cycle (17.7 hr v. 128.4 hr). The cell proliferation characteristics of the culture are further discussed in reference to both cell growth and feeding schedules of other cell lines.