Open AccessPROPERTIES OF THE H‐4‐II‐E TUMOR CELL SYSTEM
Author(s) -
Evans M. J.,
Kovacs C. J.
Publication year - 1977
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1977.tb00291.x
Subject(s) - biology
Growth and cell proliferation kinetics of hepatoma H‐4‐II‐E and its tissue culture derivative have been studied to establish the characteristics of an in vivo—in vitro solid tumor model. The H‐4‐II‐E line, originating from the Reuber H‐35 hepatoma, can be maintained and studied either in cell culture or as a transplantable solid tumor in ACI male rats. In addition it allows for the in vitro assay of cell survival following treatment of animal tumors in situ. In vivo , hepatoma H‐4‐II‐E is a rapidly growing tumor with a mean doubling time of 49.2 hr. The cell cycle time is 39.1 hr with a cell loss factor of 0.32. Retrospective examination of tumor specimens obtained during the establishment of the H‐4‐II‐E tumor system demonstrates that both structural as well as cell population changes have occurred. The biological characteristics of the primary tumor (H‐35) and an early intermediate stage (H‐35 tc2 ) are compared with H‐4‐II‐E and the histopathological, growth and cell kinetic changes are discussed.