Open AccessSPECIFIC INHIBITION OF CELL PROLIFERATION IN THE MOUSE INTESTINE BY AN AQUEOUS EXTRACT OF RABBIT SMALL INTESTINE
Author(s) -
Sassier P.,
Bergeron M.
Publication year - 1977
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1977.tb00290.x
Subject(s) - rabbit (cipher) , small intestine , large intestine , microbiology and biotechnology , cell growth , biology , cell , in vitro , chemistry , biochemistry , computer science , computer security
An aqueous extract was prepared from the mucosa of rabbit small intestine by homogenization and centrifugation at 105,000 g. After precipitation with ammonium sulfate, the 0–50 fraction (F 1 ) and the supernatant (F 2 ) were collected, dialysed against a phosphate buffer and tested on rats in vitro and mice in vivo. The F 1 fraction was found to inhibit thymidine incorporation into rat intestinal DNA in vitro , but this effect was not found to be tissue specific (liver, kidney). Two hours after a single injection of F 1 (10 mg protein content), the uptake of tritiated thymidine was decreased in jejunal and colonic DNA in mice. This effect was maximal between 2 and 4 hr and totally reversible after 7 hr; this effect was found in neither the kidney nor the testis. A slowing of cellular migration was also noticed in the jejunum and the colon. Conversely, the F 2 fraction did not inhibit the synthesis of jejunal and colonic DNA either in vitro or in vivo. Our results suggest that the F 1 fraction of the aqueous extract of rabbit small intestine contains one or more substances which may act either on intestinal DNA synthesis or on the G 1 –S transition of the cellular cycle in the mouse intestine. This reversible and specific intestinal action appears to inhibit cell proliferation and presents several of the characteristics defining a chalone.