Open AccessNUCLEIC ACID CONTENT OF HeLa S3 CELLS DURING THE CELL CYCLE: VARIATIONS BETWEEN CYCLES
Author(s) -
Thilly W. G.,
Arkin D. I.,
Wogan G. N.
Publication year - 1977
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1977.tb00285.x
Subject(s) - cell cycle , hela , nucleic acid , dna , rna , thymidine , cell , microbiology and biotechnology , biology , biophysics , constant (computer programming) , dna synthesis , cell growth , chemistry , biochemistry , gene , computer science , programming language
ABSTRACT The process of continuous resynchronization with excess thymidine provides sufficient cell material for accurate chemical determination of DNA and RNA in HeLa S3 cells at hourly intervals during the cell cycle. Total DNA is constant during the non‐S phase portion of the cell cycle but varies widely among cycles of synchronous growth. Total cellular RNA content increases linearly in the G 1 phase and accelerates to a higher linear rate of accumulation, which remains constant during most of the S and G 2 phases. The ratios of early and late cycle rates of RNA accumulation are not constant among cycles.