Open AccessRELATIONSHIP OF NUTRITIONAL FACTORS TO IN VITRO TUMOR CELL GROWTH AND CYTOTOXICITY PRODUCED BY CYTOSINE ARABINOSIDE
Author(s) -
Momparler Richard L.,
Goodman Joel
Publication year - 1977
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1977.tb00138.x
Subject(s) - cytotoxic t cell , isoleucine , cytotoxicity , biology , in vitro , biochemistry , cell growth , fibrosarcoma , amino acid , cell cycle , cell , chemistry , microbiology and biotechnology , leucine , genetics
The in vitro relationship between nutritional factors, proliferative status of tumor cells, and the cytotoxic action of cytosine arabinoside (ara‐C) was investigated. The reduction in the concentration of only one essential amino acid, L‐isoleucine, in the growth medium of A(T 1 )Cl‐3 hamster fibrosarcoma cells decreased DNA synthesis in this cell population and slowed the rate of progression of G 1 phase cells into S phase of the cell cycle. The complete omission of isoleucine from the growth medium blocked the progression of G 1 phase cells into S phase and prevented the cytotoxic action of ara‐C. The addition of isoleucine to the isoleucine‐deprived cells permitted these cells to enter the S phase and restored their sensitivity to the cytotoxic action of ara‐C. When G 1 phase cells were placed in a medium containing reduced levels of all the amino acids and vitamins there was a prolongation of the G 1 phase. Since medium with low levels of amino acids produced a delay in the entry of G 1 phase cells into the S phase, the time interval in which these cells were most sensitive to the cytotoxic action of ara‐C was different for G 1 phase cells placed in medium with adequate levels of all the amino acids. These in vitro data indicate that nutritional factors can markedly effect the proliferation of tumor cells and the cytotoxic action of ara‐C.