Open AccessREGENERATIVE PROLIFERATION OF MOUSE EPIDERMAL CELLS FOLLOWING ADHESIVE TAPE STRIPPING
Author(s) -
Clausen O. P. F.,
Lindmo T.
Publication year - 1976
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1976.tb01307.x
Subject(s) - mitosis , cell cycle , epidermis (zoology) , colcemid , population , biology , flow cytometry , cell , cell division , microbiology and biotechnology , biophysics , chemistry , andrology , anatomy , biochemistry , medicine , environmental health
Abstract The proliferating cells of mouse epidermis (basal cells) can be separated from the non‐proliferating cells (differentiating cells) (Laerum, 1969) and brought into a mono‐disperse suspension. This makes it possible to determine the cell cycle distributions (e.g. the relative number of cells in the G^ S and (G 2 + M) phases of the cell cycle) of the basal cell population by means of micro‐flow fluorometry. To study the regenerative cell proliferation in epidermis in more detail, changes in cell cycle distributions were observed by means of micro‐flow fluorometry during the first 48 hr following adhesive tape stripping. 3 H‐TdR uptake (LI and grain count distribution) and mitotic rate (colcemid method) were also observed. An initial accumulation of G 2 cells was observed 2 hr after stripping, followed by a subsequent decrease to less than half the control level. This was followed by an increase of cells entering mitosis from an initial depression to a first peak between 5 and 9 hr which could be satisfactorily explained by the changes in the G 2 pool. After an initial depression of the S phase parameters, three peaks with intervals of about 12 hr followed. The cells in these peaks could be followed as cohorts through the G 2 phase and mitosis, indicating a partial synchrony of cell cycle passage, with a shortening of the mean generation time of basal cells from 83‐3 hr to about 12 hr. The oscillations of the proportion of cells in G 2 phase indicated a rapid passage through this cell cycle phase. The S phase duration was within the normal range but showed a moderate decrease and the Gj phase duration was decreased to a minimum. In rapidly proliferating epidermis there was a good correlation between change in the number of labelled cells and cells with S phase DNA content. This shows that micro‐flow fluorometry is a rapid method for the study of cell kinetics in a perturbed cell system in vivo.