Open AccessEHRLICH ASCITES TUMOR (EAT) CHALONE EFFECTS ON NASCENT DNA SYNTHESIS AND DNA POLYMERASE ALPHA AND BETA
Author(s) -
Nakai George S.
Publication year - 1976
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1976.tb01305.x
Subject(s) - dna polymerase , dna synthesis , microbiology and biotechnology , dna , primer (cosmetics) , polymerase , biology , dna clamp , dna polymerase ii , biochemistry , thymidine , dna polymerase beta , chemistry , polymerase chain reaction , dna repair , reverse transcriptase , gene , organic chemistry , base excision repair
EAT chalone effects on nascent DNA synthesis and DNA polymerase were examined. Concentration related inhibition of 3 H‐thymidine ( 3 H‐TdR) incorporation into EAT cell DNA was noted over a chalone range of 50–200 μg/ml. RNA synthesis was not affected, but protein synthesis decreased an average of 82% during 3 hr. Nascent DNA pulse‐labeled for 2 min was normally incorporated into bulk DNA in the presence of chalone, but crude α and β‐polymerase activities were inhibited. Crude DNA polymerase from C 3 H mouse kidney and spleen was also partially inhibited by EAT chalone, suggesting non‐specific inhibition of DNA polymerase. Preincubation studies of chalone with crude EAT DNA polymerase or ‘gapped’ DNA primer had no effect on chalone activity. Chalone may control mitotic activity by inhibiting α‐ and β‐polymerase activity, thereby decreasing nascent DNA synthesis. Nascent DNA is incorporated normally into bulk DNA in the presence of chalone, indicating that DNA ligase is not inhibited.