Open AccessHYDROXYUREA: USE IN DETERMINING THE DURATION OF G 2 IN TETRAHYMENA
Author(s) -
WORTHINGTON D. H.,
NACHTWEY D. S.
Publication year - 1976
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1976.tb01297.x
Subject(s) - tetrahymena pyriformis , tetrahymena , thymidine , fraction (chemistry) , cell division , population , cell cycle , biology , division (mathematics) , exponential growth , generation time , cell , microbiology and biotechnology , chemistry , biochemistry , chromatography , physics , medicine , mathematics , in vitro , arithmetic , environmental health , quantum mechanics
Observation of division of individual cells in microdrops, plus autoradiographic studies using tritiated thymidine and standard cell cycle analysis techniques, reveal that hydroxyurea (10 DIM) reversibly arrests the normal progression of exponentially growing Tetrahymena pyriformis through the initial 92 % of S‐phase while not affecting cells in the terminal 8 % and in G 2 and division. Thus the fraction of the population of cells that is in G 2 can be approximately determined by the fraction of the population able to divide in the presence of hydroxyurea. This fraction can be related to the approximate duration of G 2 by calculations which compensate for the age gradient.