Open AccessSTUDY ON THE PROLIFERATIVE STATE OF HAEMOPOIETIC STEM CELLS (CFU)
Author(s) -
NEČAS E.,
POŇKA P.,
NEUWIRT J.
Publication year - 1976
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1976.tb01270.x
Subject(s) - haematopoiesis , stem cell , transplantation , andrology , biology , microbiology and biotechnology , cell growth , colony forming unit , cfu gm , cell division , cell , immunology , chemistry , medicine , biochemistry , genetics , bacteria
Hydroxyurea was used to study the proliferation rate of haemopoietic stem cells (CFUJ in normal mice, after irradiation or transplantation into irradiated recipients. It was demonstrated that the proliferation rate of endogenous CFU S (endo‐CFU,) and exogenous CFU S (exo‐CFU s ) are identical. After irradiation (650 R) the surviving endo‐CFU s begin to proliferate immediately. By contrast exo‐CFU, transplanted into the irradiated recipient mouse (850 R), begin to proliferate only after about 30 hr. However, injection of isoproterenol (which stimulates adenyl cyclase) or dibutyryl cyclic adenosine 3′,5′‐monophosphate shortly after marrow cell graft, triggers the transplanted CFU S into cell cycle as shown by an almost immediately increased sensitivity to hydroxyurea. Isoproterenol is capable of inducing DNA synthesis also in stem cells of normal mice but it takes about 20 hr before CFU, become to be increasingly sensitive to hydroxyurea.