Open AccessPREFERENTIAL LABELING OF RAT LYMPHOCYTES WITH A RAPID RATE OF TURNOVER BY TRITIATED DEOXYCYTIDINE
Author(s) -
Amano M.,
Everett N. B.
Publication year - 1976
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1976.tb01264.x
Subject(s) - thymidine , thymine , tritium , dna , cytosine , pyrimidine , lymphocyte , biochemistry , deoxycytidine , dna synthesis , biology , microbiology and biotechnology , chemistry , immunology , genetics , physics , chemotherapy , gemcitabine , nuclear physics
Lymphocytes in thymic cortex and germinal centers of lymphoid tissues are labeled intensely with generally labeled tritiated deoxycytidine [G‐ 3 H]dCyd whereas they are weakly labeled with methyl tritiated deoxythymidine [methyl‐ 3 H]dThd of the same specific activity, not only by single injection but also by an intensive injection schedule. [G‐ 3 H]dCyd can be used to label short‐lived lymphocytes strongly, although not specifically. The distribution patterns of labeled lymphocytes were different depending on the injection schedules of [G‐ 3 H]dCyd. [G‐ 3 H]dCyd can be used as a precursor molecule for cytosine and also thymine found in DNA. The ratios of radioactive thymine to cytosine measured biochemically on DNA extracted from radioactive lymphocytes labeled by the various schedules indicate strongly that short‐ and long‐lived lymphocyte populations have different abilities to utilize pyrimidine nucleosides for DNA synthesis.