Open AccessCELL KINETICS METHODS FOR CLINICAL USE: ASSESSMENT OF AUTORADIOGRAPHY AND 125 I‐IODO‐DEOXYURIDINE WITH MOUSE EHRLICH TUMOUR
Author(s) -
Jenkinson I. S.,
Shuter B. J.,
Wright P. N. M.
Publication year - 1975
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1975.tb01496.x
Subject(s) - kinetics , deoxyuridine , radiochemistry , cell , chemistry , microbiology and biotechnology , biochemistry , biology , dna , physics , quantum mechanics
Superficial Ehrlich tumours in mice were used to assess how much information on cell kinetics could be obtained from only the simplest techniques of autoradiography, and in situ monitoring of 125 I‐iodo‐deoxyuridine. These techniques were selected as being readily applicable to clinical situations. Intradermal tumours were studied from the earliest stages of rapid growth to large slow growing tumours with necrotic cores, as well as tumours undergoing regression. For comparison, intramuscular tumours were studied with systemic injections of radioactive DNA precursors. It was found that extensive information on cell production and loss rates was obtained from a single injection of tritiated thymidine followed by a single biopsy, or alternatively in vitro labelling of one minute biopsy specimen, and measurements of only the labelling index, together with a knowledge of the tumour's growth characteristics. Following a single localized injection of 125 I‐iodo‐deoxyuridine, the loss rate of radioactivity measured in situ for a period of about 1 week provided an index of cell loss rate from the tumours.