Open AccessTHE INFLUENCE OF A GRANULOCYTIC INHIBITOR(S) ON HEMATOPOIESIS IN AN IN VIVO CULTURE SYSTEM
Author(s) -
MacVittie Thomas J.,
McCarthy Kenneth F.
Publication year - 1975
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1975.tb01241.x
Subject(s) - haematopoiesis , in vivo , progenitor cell , granulocyte , biology , population , cell growth , bone marrow , microbiology and biotechnology , immunology , cell culture , stem cell , andrology , chemistry , biochemistry , medicine , genetics , environmental health
The in vivo diffusion chamber (DC) technique for mouse marrow culture was used to determine the effect of a granulocyte inhibitor on the proliferation of the pluri‐potent stem cell (CFU‐s) and the granulocyte progenitor cell (CFU‐c). Granulocyte conditioned medium was injected intraperitoneally into mice bearing DCs during the initial 48 hr of culture. The early injection of inhibitor resulted in a significantly reduced number of granulocytic progeny formed within the DCs while there was no growth inhibition of mouse fibroblasts cultured under identical conditions. The reduced cell production was due in part to a significant reduction in the self‐renewal rate of the CFU‐c while no apparent direct effect was observed upon the growth of the CFU‐s within the same cultures. These data suggest that the granulocytic inhibitor(s) acted to reduce proliferation within the CFU‐c population and thereby diminished the amplification potential inherent in the initial cell inoculum.