Open AccessPOLYMETHACRYLIC ACID: INDUCTION OF LYMPHOCYTOSIS AND TISSUE DISTRIBUTION
Author(s) -
Ross W. M.,
Martens A. C.,
Bekkum D. W. van
Publication year - 1975
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1975.tb01233.x
Subject(s) - lymphocytosis , spleen , lymph , mesenteric lymph nodes , bone marrow , population , lymphocyte , perfusion , medicine , immunology , endocrinology , pathology , chemistry , environmental health
Polymethacrylic acid (PMAA) induces up to a three‐fold increase in the lymphocyte population of peripheral blood in rats, goats and calves after intravenous administration. Other routes of administration are less effective. A maximum lymphocytosis is achieved after 3 hr with all doses in excess of 30 mg PMAA/kg body weight; over the next few hours the lymphocyte level declines to normal. Granulocytes increase steadily for the first 7 hr before declining. Multiple doses of PMAA 2 hr apart failed to maintain or significantly alter the lymphocytosis. PMAA was labelled with 125 I and 14 C, and was traced to various sites in the rat. The greatest accumulation of radioactivity was in the spleen, lungs, liver, kidney, adrenals and mesenteric lymph nodes (with 14 C‐PMAA). The accumulation appeared more specific for spleen and lymph nodes since there was only a small loss of activity following removal of blood by whole body perfusion. This supports previous findings indicating that these two tissues play a major role in the development of lymphocytosis. Accumulation in the bone marrow may be indicative of stem cell mobilization. The results are discussed in terms of the lymphocytosis‐inducing mechanism and the site of action of PMAA and the possible clinical application to ECIB therapy is considered.