Open AccessRELATIONSHIPS BETWEEN CELL CYCLE DURATION, S‐PERIOD AND NUCLEAR DNA CONTENT IN ERYTHROBLASTS OF FOUR VERTEBRATE SPECIES
Author(s) -
Grosset Lise,
Odartchenko Nicolas
Publication year - 1975
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1975.tb01209.x
Subject(s) - biology , nuclear dna , ploidy , thymidine , dna , cell cycle , vertebrate , mitosis , period (music) , dna synthesis , microbiology and biotechnology , cell , genetics , gene , mitochondrial dna , physics , acoustics
Erythroblasts of four vertebrate species ( Triturus cristatus, Rana esculenta, Lacerta viridis and Gallus domesticus ) differing markedly in their nuclear diploid DNA content, are used to study a possible relationship between cell cycle duration and DNA content. DNA is determined cytophotometrically and fluorometrically. The cell cycle is analysed by evaluating labelled mitoses after an injection of tritiated thymidine and also by double labelling with 14 C‐ and 3 H‐thymidine. A direct but non‐linear relationship is demonstrated between DNA content of erythroblast nuclei and the duration of DNA‐synthesis.