Open AccessACTIVITY OF TUBULOSINE ON THE KINETICS OF ROOT MERISTEM CELL POPULATION
Author(s) -
Pareyre Claude,
Deysson Guy
Publication year - 1975
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1975.tb01208.x
Subject(s) - labelling , mitosis , mitotic index , thymidine , meristem , population , cell cycle , biophysics , biology , kinetics , cell , chemistry , root tip , microbiology and biotechnology , biochemistry , botany , dna , physics , demography , quantum mechanics , sociology , gene
The action of tubulosine on the mitotic cycle was studied using continuous labelling with tritiated thymidine. This alkaloid provokes a lengthening of the G 1 and S phases and a blocking of G 2 is totally reversible when the treatment is followed by recovery in normal medium. At a dose of tubulosine which induces a reversible mitostasis in the shortest possible time the lengthening of the phases of the cell cycle was estimated by three different techniques: labelled mitoses for the determination of G 2 ; labelling intensity for the determination of S; binucleate cells for the determination of T, and an original technique using labelling index of binucleate cells for the determination of G 1 . The limits of the technique of labelled mitosis together with the interest of the technique aiming at the direct determination of G 1 in the case of a perturbed cycle are then discussed.