Open AccessRECOVERY OF PROLIFERATING HAEMOPOIETIC PROGENITOR CELLS AFTER KILLING BY HYDROXYUREA
Author(s) -
Hodgson G. S.,
Bradley T. R.,
Martin R. F.,
Sumner M.,
Fry P.
Publication year - 1975
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1975.tb01206.x
Subject(s) - spleen , progenitor cell , bone marrow , andrology , biology , microbiology and biotechnology , doubling time , peripheral blood , cfu gm , immunology , stem cell , medicine , cell , biochemistry
Two doses of 1 mg/g of hydroxyurea (HU), injected 7 hr apart into irradiated mice in which CFU‐S were proliferating during marrow regeneration, killed about 90% of CFU‐S. This same dose regime injected into normal female mice, with non‐proliferating CFU‐S killed 92 % of CFU‐C, 99 % of ESC and only 30 % of CFU‐S. One day after the treatment CFU‐S had decreased to 50 % and remained at about this level for a further day then returned to normal values. In spleen the increase in CFU‐S was delayed by a day and showed a marked overshoot. During the period that CFU‐S were decreased in number they were actively proliferating. Marrow CFU‐C recovered in an exponential manner with a doubling time of 16 hr. Spleen CFU‐C recovered 1 day later than marrow and showed a pronounced overshoot. ESC recovered very rapidly with doubling time of 5 hr. The changes in 59 Fe incorporation into RBC, and the peripheral blood picture, were a delayed reflection of the changes in ESC and CFU‐C.