Open AccessCHANGES IN CELL PROLIFERATION KINETICS OCCURRING DURING THE LIFE HISTORY OF MONOLAYER CULTURES OF A MOUSE TUMOUR CELL LINE
Author(s) -
TWENTYMAN P. R.,
WATSON J. V.,
BLEEHEN N. M.,
ROWLES P. M.
Publication year - 1975
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1975.tb01205.x
Subject(s) - labelling , doubling time , cell cycle , cell culture , cell division , biology , cell , kinetics , population , plateau (mathematics) , cell growth , exponential growth , monolayer , andrology , microbiology and biotechnology , immunology , chemistry , biochemistry , medicine , genetics , mathematics , mathematical analysis , physics , environmental health , quantum mechanics
Following inoculation of monolayer cultures of EMT6 mouse tumour cells at 10 5 cells, a short lag is followed by 3 days of exponential growth with a population doubling time of 12 hr. A plateau cell number is reached between days 4 and 5 and is maintained for at least 8 days. During exponential growth, the pulse 3 H‐TdR labelling index is 55–60%, all cells are in cycle, and the median cycle time is 11–12 hr. For the first 3 days of plateau phase, the labelling index is about 25 % and there is considerable cell loss. The cell cycle is 32–40 hr, and S‐phase is very long. Later in plateau phase, the labelling index falls to <2 % and there is little cell loss. The changes in kinetics occurring in EMT6 cultures are discussed with reference to reported changes occurring in other cell lines.