Open AccessAUTOSYNCHRONIZATION OF RAT LIVER CELLS WITH ENDOGENOUS CORTICOSTERONE AFTER PARTIAL HEPATECTOMY
Author(s) -
DesserWiest L.
Publication year - 1975
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1975.tb01201.x
Subject(s) - corticosterone , medicine , endocrinology , adrenalectomy , tyrosine aminotransferase , hepatectomy , hepatocyte , biology , chemistry , hormone , enzyme , biochemistry , in vitro , enzyme inducer , surgery , resection
After adrenalectomy in adult male rats 3 H‐TdR incorporation into the liver parenchymal cells is increased 4–8 times and the mitotic index rises from 0–31 % to 1–3%; this is inhibited by corticosterone. After hepatectomy the serum corticosterone level increases from 18 μg/100 ml to 57 μg/100 ml. The corticosterone binding capacity of the serum declines from 2–06 to 0–17. The activity of tyrosine transaminase doubles, whereas the incorporation of 3 H‐TdR into the liver cells is decreased by a factor of 5–7. Thereafter the binding capacity increases again and reaches, 24 hr after operation, a value of 3–82. The tyrosine transaminase activity and the serum corticosterone content return to normal. 3 H‐TdR incorporation, however, increases by a factor of 7‐7 of the initial value. We concluded that in the first few hours after partial hepatectomy corticosterone blocks the liver cells in G 1 and an accumulation of the cells occurs at this cell cycle phase. Folio wing the binding of the corticosterone by serum proteins a little later the liver cells enter the S‐phase synchronously.