Open AccessHAEMOPOIETIC STEM CELL (CFU) KINETICS IN THE CULTURE
Author(s) -
Chertkov J. L.,
Samoyliina L.,
Rudneva Nina A.,
Rakcheev A. M.,
Kondratenko a F.
Publication year - 1974
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1974.tb00906.x
Subject(s) - haematopoiesis , stem cell , biology , bone marrow , microbiology and biotechnology , immunology , cell culture , andrology , medicine , genetics
Haemopoiesis continued for over 2 months in organ culture of embryonal mouse liver, and haemopoietic stem cells (CFU s ) capable of DNA‐synthesis were found in it all that time. Between the 10th and 40th day the number of stem cells in the culture was sustained in a steady state. Both in normal and in regenerating adult bone marrow haemopoiesis ceased within a short time in the culture. Induction of proliferation in haemopoietic stem cells combined with undamaged or improved micro‐environment resulted in a little better maintenance of CFU s in the adult bone marrow culture, The results are discussed in the light of current concepts of haemopoietic stem cell regulation.