Open AccessESTIMATION OF THE MEAN AND VARIANCE OF CYCLE TIMES IN CINEMICROGRAPHICALLY RECORDED CELL POPULATIONS DURING BALANCED EXPONENTIAL GROWTH
Author(s) -
Jagers Peter,
Norrby Klas
Publication year - 1974
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1974.tb00900.x
Subject(s) - mitosis , doubling time , anaphase , cytokinesis , cell cycle , biology , exponential growth , variance (accounting) , population , exponential function , cell , microbiology and biotechnology , mathematics , andrology , cell division , genetics , mathematical analysis , economics , demography , accounting , sociology , medicine
The mathematical and statistical problem of estimating the mean and variance of cell cycle times from cinemicrographically observed durations until mitosis (cytokinesis at late anaphase), disintegration, collision (cells superimposing each other) or emigration (moving out of the field of vision) of randomly chosen individual cells in a population in balanced exponential growth is treated. The resulting formulae are simple and considerably reduce the average cell observation time. They are applied to two normal human foetal cell lines and their SV40‐transformed counterparts. One result is that the latter seem to have longer cycle times in spite of their shorter doubling times. Another result is that the cell mobility seems somewhat increased in the transformed populations. This corroborates earlier findings, indicating that the extent of cell loss, rather than the length of cycle times, may play a decisive role for the (net) doubling time of cultivated cell populations.