Open AccessDIFFERENT ACTION OF SUICIDAL DOSES OF TRITIATED THYMIDINE AND HYDROXYUREA ON MURINE HAEMOPOIETIC CELLS
Author(s) -
Schreml W.,
Bock O.,
Bock E.,
Heit W.,
Kubanek B.
Publication year - 1974
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1974.tb00435.x
Subject(s) - thymidine , spleen , bone marrow , dna synthesis , dna , deoxyuridine , stem cell , biology , microbiology and biotechnology , pharmacology , chemistry , immunology , biochemistry , genetics
In order to gather information on the factors that cause the different action of suicidal doses of tritiated thymidine ( 3 H‐TdR) and of hydroxyurea on murine stem cells, the incorporation of 3 H‐TdR into DNA of bone marrow and spleen cells has been studied. Continuous death of labelled cells after suicidal 3 H‐TdR is indicated by a more pronounced decline of total DNA‐bound radioactivity in bone marrow and spleen cells compared to that in control animals which had received tracer doses of 3 H‐TdR. Extensive and rapid loss of DNA‐bound radioactivity occurred in 3 H‐TdR labelled animals after hydroxyurea treatment indicating an instantaneous and highly effective killing of labelled cells. After double labelling of DNA with 3 H‐TdR and 125 iodo‐deoxyuridine ( 125 I‐UdR), the decline of the ratio of DNA‐bound 125 I to DNA‐bound 3 H after suicidal 3 H‐TdR indicates prolonged tritium reutilization. Following hydroxyurea, reutilization was completed within the first 12 hr after drug administration. These findings explain in part the slow recovery of different stem cell compartments after suicidal 3 H‐TdR on the basis of protracted tritium reutilization as compared to the fast recovery which follows the rapid action of hydroxyurea.