Open AccessEXPRESSION OF CONGENITAL DEFECTS IN THE HAEMOPOIETIC MICRO‐ENVIRONMENT: PRE‐NATAL ERYTHROPOIESIS IN ANAEMIC ‘STEEL’( Sl j Sl j ) MICE
Author(s) -
Cole R. J.,
Tarbutt R. G.,
Cheek E. M.,
White S. L.
Publication year - 1974
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1974.tb00430.x
Subject(s) - erythropoiesis , erythropoietin , in vitro , andrology , fetus , medicine , biology , microbiology and biotechnology , chemistry , endocrinology , anemia , biochemistry , pregnancy , genetics
The production of red cells by the livers of congenitally anaemic Sl j Sl j foetal mice is reduced, so that the red cell concentration reaches only 0.4 × 10 9 /ml, compared to 2.3 × 10 9 /ml in normal littermates. The deficiency in haemoglobin concentration is not so severe (3 g/100 ml compared to 10 g/100 ml), because Sl j Sl j red cells are macrocytic and normochromic. The number of erythroblasts in the livers of Sl j Sl j foetuses is considerably less than in normal littermates, but the ratio of early erythroblasts to late erythroblasts is not altered. The cell cycles of Sl j Sl j and normal early erythroblasts are similar, but the cycle time of Sl j Sl j late erythroblasts is longer than that of the corresponding normal cells. Sl j Sl j foetal liver cells have a restricted response to erythropoietin in vitro in terms of haem synthesis, whereas foetal liver cells of normal littermates are highly responsive until the 16th day of gestation. Mixtures of late 16 day Sl j Sl j and normal foetal liver cells (both non‐responsive alone) respond to erythropoietin in vitro .