Open AccessAN AUTOMATIC SYNCHRONIZER FOR SUSPENSION CULTURE OF L5178Y CELLS
Author(s) -
Okada S.,
Shinohara K.
Publication year - 1974
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1974.tb00412.x
Subject(s) - colcemid , thymidine , synchronizer , mitosis , suspension culture , population , suspension (topology) , biology , synchronization (alternating current) , chemistry , microbiology and biotechnology , computer science , cell culture , mathematics , genetics , dna , medicine , programming language , combinatorics , topology (electrical circuits) , environmental health , homotopy , pure mathematics
The excess thymidine‐colcemid synchronization method for mouse leukemia L5178Y was automated by deleting the removal of excess thymidine. The synchronizer adds thymidine and colcemid at predetermined times and makes the population accumulated at mitosis available at a designated time. The apparatus is inexpensive to build, easily operated and applicable to large volumes of a static suspension culture.