Open AccessCLEARANCE RATE OF EXOGENOUS 3 H‐THYMIDINE FROM THE PLASMA OF PREGNANT RHESUS MONKEYS
Author(s) -
Nowakowski Richard S.,
Rakic Pasko
Publication year - 1974
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1974.tb00411.x
Subject(s) - thymidine , clearance rate , kinetics , tritium , chemistry , biology , endocrinology , medicine , andrology , dna , microbiology and biotechnology , biochemistry , physics , quantum mechanics , nuclear physics
The plasma clearance rate of 3 H‐TdR was determined by thin‐layer chromatography of samples from pregnant rhesus monkeys who had received a single intravenous injection of 3 H‐TdR. At 10 min after the injection the circulating levels of 3 H‐TdR had fallen to less than 0.2% of the initial theoretical distribution of the label. In general the plasma clearance rate can be described by a ‘double exponential’curve with half‐lives of 1 min and 20 min. Thus, 3 H‐TdR disappears from the circulation of the pregnant monkey more rapidly than it does from the bloodstream of rodents, which correlates with previously detected interspecies differences in autoradiographic labeling of neurons. This means (1) that higher doses of 3 H‐TdR must be used for autoradiographic labeling of monkey tissues and (2) that the monkey may be useful for studying cell kinetics because it has a short pulse labeling of the DNA.