Open AccessCOMPARATIVE PROLIFERATIVE KINETICS OF CELLS FROM NORMAL HUMAN EPIDERMIS AND BENIGN EPIDERMAL HYPERPLASIA (PSORIASIS) IN VITRO
Author(s) -
Chopra D. P.,
Flaxman B. A.
Publication year - 1974
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1974.tb00400.x
Subject(s) - psoriasis , epidermis (zoology) , in vitro , hyperplasia , thymidine , kinetics , mitosis , human skin , dna synthesis , pathology , biology , chemistry , microbiology and biotechnology , immunology , medicine , anatomy , biochemistry , physics , genetics , quantum mechanics
Proliferation kinetics of epidermal cells from normal human skin and lesions of psoriasis (benign epidermal hyperplasia) were studied in vitro . Epithelial out‐growths were obtained from skin explants and the cell cycle studied using the conventional method of following two successive curves of labeled mitoses after an initial pulse with 3 H thymidine. The length of T c was 59 hr and 53.5 hr respectively for normal and psoriatic cells. The shorter T c for psoriasis was due to a shorter duration of S. The growth fraction was 66% and 74% for normal and psoriatic cells respectively as determined by continuous labeling with 3 H thymidine. Under the conditions of the present experiments, therefore, normal and psoriatic epidermal cells showed no significant difference in proliferative capacity.