Open AccessEFFECTS OF ISOGENIC BONE MARROW CELL DOSE ON ERYTHROKINETICS OF LETHALLY X‐IRRADIATED MICE
Author(s) -
Smith L. H.,
Goodman Joan W.,
Hodgson George S.
Publication year - 1974
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1974.tb00397.x
Subject(s) - erythropoiesis , bone marrow , haematopoiesis , spleen , cell , andrology , hematocrit , biology , compartment (ship) , immunology , blood cell , red cell , regeneration (biology) , irradiation , cell growth , microbiology and biotechnology , medicine , stem cell , endocrinology , anemia , biochemistry , oceanography , physics , geology , nuclear physics
The effect of isogenic bone marrow cell dose on several parameters of hemopoietic regeneration in lethally X‐irradiated mice was studied. The appearance of 59 Fe in red blood cells (RBC) and spleen, the hematocrits, and the spleen weights were measured at various times after irradiation and the injection of 10 5 , 10 6 , 10 7 or 10 8 marrow cells. Results show that with increasing cell dose, regeneration of erythropoiesis was detectable earlier, proceeded at a faster rate, reached a peak more quickly, and subsided to normal levels sooner. The initial spleen‐weight growth rate was independent of cell dose, although the onset of growth was earlier the higher the dose. The maximum rate of RBC production was attained earlier the larger the cell dose and was a decreasing function of the hematocrit at that time, which in itself was an increasing function of cell dose. These data support the concept of a proportional model for control of erythropoiesis as described by Hodgson (1970). The present data are interpretable in terms of an A‐ and B‐cell model for erythropoiesis as suggested by Lajtha, Gilbert & Guzman (1971). This model postulates a compartment of undifferentiated multipotent A cells and a compartment of differentiating erythropoietic B cells capable of amplification according to the need for RBC. Application of the present data to the A‐B cell model generates a tentative description of the changes in kinetics of amplification in the B‐cell compartment as the size of the A‐cell base is changed.