Open AccessCELL KINETICS IN MOUSE THYMUS STUDIED BY SIMULTANEOUS USE OF 3 H‐THYMIDINE AND COLCHICINE
Author(s) -
Borum Kirstine
Publication year - 1973
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1973.tb01645.x
Subject(s) - colcemid , colchicine , mitosis , cell division , thymidine , cell , biology , microbiology and biotechnology , dna synthesis , chemistry , dna , biochemistry , genetics
Following an injection of 3 H‐thymidine to mice there is no initial incorporation in small thymocytes, only in larger ones. In the course of time small thymocytes aquire the label. Whether the delayed uptake in small thymocytes is due to a direct cell to cell transfer of labelled nuclear material from inititally labelled larger cells to small thymocytes, or whether it is due to small thymocytes being formed from larger cells by mitotic division was investigated by the administration of Colcemid® immediately after one injection of 3 H‐thymidine. In the absence of cell division no labelled small thymocytes appeared with time. This finding does not support the idea of a cell to cell transfer of DNA; it rather lends support to the view that small thymocytes arise by mitotic division of larger cells in the thymus. During the treatment with Colcemid® the migration of cells took place from peripheral to central cortex just like under normal conditions.