Open AccessTHE PROLIFERATION OF LYMPHOID CELLS IN GUINEA‐PIG BONE MARROW
Author(s) -
Miller S. C.,
Osmond D. G.
Publication year - 1973
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1973.tb01615.x
Subject(s) - basophilic , bone marrow , biology , in vivo , thymidine , cytoplasm , dna synthesis , microbiology and biotechnology , in vitro , efflux , cell cycle , cell , immunology , pathology , biochemistry , medicine , genetics
A double isotope DNA labelling method has been used to determine the duration of DNA synthesis (S) in bone marrow lymphoid cells classified by their nuclear diameters in smears. Incorporation of 3 H‐thymidine was confined almost entirely to marrow lymphoid cells of 8·0‐15·0 μm nuclear diameter (large lymphoid cells). After exposure to 3 H‐thymidine in vivo and 14 C‐thymidine 40‐104 min later in vitro , the proportion of cells labelled with 3 H alone to those labelled with 14 C(± 3 H) in radioautographic smears, plotted against time indicated the efflux from S per hour. Collectively, 28·3 ± 1·1% of all large lymphoid cells were in S and the efflux from S was 15·1% per hour. With decreasing cell size (nuclear diameter) the efflux fell progressively from 28·3% per hour (11·0 μm) to 9·2% per hour (8·0‐8·9 μm) and the proportion of cells in S declined from 54·9 ± 2·3% to 14·8 ± 1·6%. Influx into S, measured in vitro by reversing the sequence of isotopes, closely resembled the corresponding efflux values in vivo relative to cell size. Most DNA synthesizing marrow large lymphoid cells belonged to a subgroup with deeply basophilic cytoplasm. The results demonstrate that basophilic large lymphoid cells in the marrow are actively proliferating and have a mean S phase duration of 6·6 hr. The largest marrow lymphoid cells (11·0 μm) proliferate most rapidly (S phase, 3·5 hr; maximum cell cycle time, 6·4 hr) while S duration is prolonged progressively to 10·9 hr for the smaller cells (8·0‐8·9 μm).