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open-access-imgOpen AccessAUTORADIOGRAPHIC CONFIRMATION OF THE MITOTIC DIVISION OF EVERY MOUSE JEJUNAL CRYPT CELL LABELLED WITH 3 H‐THYMIDINE
Author(s)
Burholt D. R.,
Schultze B.,
Maurer W.
Publication year1973
Publication title
cell proliferation
Resource typeJournals
PublisherBlackwell Publishing Ltd
ABSTRACT The question was investigated of whether for crypt epithelia of the jejunum of the mouse all cells labelled after a single injection of 3 H‐TdR subsequently divide or whether cells exist in the crypt which synthesize metabolic DNA and, therefore, do not undergo division after labelling. A double labelling experiment was performed with a first injection of 3 H‐TdR followed 1 hr later by an injection of 14 C‐TdR. Then from double emulsion autoradiographs of isolated squashed crypts the number of 3 H‐only, 14 C‐only and double labelled cells and mitoses were counted. The double labelling produced a narrow, 1 hr wide sub‐population of 3 H‐only labelled cells. This subpopulation of S cells completed its division before labelled cells were lost from the crypts by migration onto the villi. The results showed that this subpopulation of 3 H‐only cells completely doubled within 3 hr and then remained constant through 6 hr. From this result it was concluded that every cell labelled after a single injection of 3 H‐TdR divides. From the same autoradiographs the flow rate through the end of mitosis was measured. From the flow rate and the mitotic index a mitotic duration of 0·5 hr was determined. The agreement of this measured mitotic time with the value calculated from the labelling index, mitotic index and S duration is also strong evidence that every labelled cell divides. Both experiments show that the intestinal crypt does not contain cells synthesizing metabolic DNA.
Subject(s)biochemistry , biology , cell , cell division , crypt , dna , dna synthesis , endocrinology , environmental health , labelling , medicine , microbiology and biotechnology , mitosis , mitotic index , population , thymidine
Language(s)English
SCImago Journal Rank1.647
H-Index74
eISSN1365-2184
pISSN0960-7722
DOI10.1111/j.1365-2184.1973.tb01612.x

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