Open AccessLOCAL AND SYSTEMIC CONTROL OF GRANULOCYTIC AND MACROPHAGE PROGENITOR CELL REGENERATION AFTER IRRADIATION
Author(s) -
Chan S. H.,
Metcalf D.
Publication year - 1973
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1973.tb01607.x
Subject(s) - bone marrow , progenitor cell , regeneration (biology) , macrophage , stromal cell , spleen , in vitro , biology , immunology , stem cell , chemistry , microbiology and biotechnology , cancer research , biochemistry
Agar cultures of C 57 BL bone marrow cells were used to determine colony stimulating factor (CSF) and serum CSF‐inhibitor levels in C 57 BL and BALB/c mice following irradiation. Whole‐body irradiation caused an acute, dose‐dependent, rise in serum CSF levels and fall in CSF‐inhibitor levels. The regeneration of granulocytic and macrophage progenitor cells ( in vitro CFCs) in the femur after 250 rads whole‐body irradiation was preceded or paralleled by a fall in serum CSF‐inhibitors and a dramatic rise in the capacity of bone‐adherent cells in the marrow (‘stromal cells’) to produce material with colony‐stimulating activity. No comparable changes were observed in the activity of marrow haemopoietic cells during regeneration or in the lungs or spleen. A similar rise in the activity of bone‐adherent cells was observed in shielded femurs during regeneration of in vitro CFCs. Regeneration of granulocytic and macrophage progenitor cells following irradiation may be regulated by fluctuations in circulating CSF‐inhibitor levels and local production of CSF within the marrow cavity.