Open AccessKINETICS OF MURINE HAEMOPOIETIC CELL PROLIFERATION IN DIFFUSION CHAMBERS
Author(s) -
Bøyum Arne,
Breivik Harald
Publication year - 1973
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1973.tb01599.x
Subject(s) - kinetics , granulocyte , macrophage , diffusion , bone marrow , microbiology and biotechnology , cell growth , chemistry , immunology , cell , andrology , biology , biophysics , biochemistry , medicine , in vitro , physics , quantum mechanics , thermodynamics
Murine bone marrow cells were cultured in cell impermeable diffusion chambers in the abdominal cavities of mice. The kinetics of granulocyte and macrophage formation were studied by stathmokinetic and autoradiographic techniques. During the period of most rapid growth of proliferative granulocytes, their generation time and its different phases were: t c ∽ 8 hr, t G1 ∽ 1·5 hr, t s ∽ 5·5 hr, t G2 ∽ 0·7 hr and t M ∽ 0·25 hr. The generation time of macrophages and their precursors was approximately 8 hr. Formation of macrophages was significantly reduced when chamber inoculum was increased, as judged by 3 H‐TdR labelling index.