Open AccessTHE EFFECT OF CHEMOTHERAPY ON THE GROWTH OF LEUKEMIA L1210 II. PERSISTENCE OF A NITROSOUREA‐INDUCED CHANGE IN THE GROWTH CHARACTERISTICS OF TRANSPLANT GENERATIONS
Author(s) -
DeVita Vincent T.,
Bray David A.,
Bostick Frieda,
Bagley Charles M.
Publication year - 1972
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1972.tb00384.x
Subject(s) - persistence (discontinuity) , leukemia , nitrosourea , chemotherapy , doubling time , growth inhibition , population , biology , immunology , cancer research , cell culture , medicine , genetics , geotechnical engineering , environmental health , engineering
A strain of murine leukemia L1210 was treated with subcurative doses of 1,3 bis‐(2‐chloroethyl)‐1‐nitrosourea. When the leukemia cells repopulated the abdominal cavity, aliquots were transplanted to other animals and the growth characteristics measured. A PLM curve obtained twenty‐one generations later had a different configuration than the control line with a prolongation of T s and T c . The configuration suggested that treatment with BCNU may have led to the selective growth of a population of cells with a longer T c and less variation about the mean T c . Permanent actual alteration of T c could not be excluded. Measurements of T D also appeared to be affected by the number of tumor cells present on the day of the study. Although T c was prolonged in drug‐exposed transplanted cell lines, cell loss, which may be influenced by many factors, appears to be the major factor that must be considered in alterations of doubling times in this model tumor system.