Open AccessHuman Lymphocyte Proliferation: Dna Synthesis Time
Author(s) -
Schiffer L. M.
Publication year - 1971
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1971.tb01566.x
Subject(s) - basophilic , cytoplasm , microbiology and biotechnology , lymph node , lymphocyte , biology , t lymphocyte , in vitro , pathology , immunology , medicine , biochemistry
. The DNA synthesis time ( T s ) of lymphocytes from spleens and lymph nodes of patients with Hodgkin's disease was determined by the double labeling method. 3 H‐TdR was administered in vivo and removed tissues minced in 14 C‐TdR in vitro. Lymphocytes from patients with lymphosarcoma and reticulum cell sarcoma were studied in a similar manner. Lymphocytes were divided into A cells, small with non‐basophilic cytoplasm, B cells, small with basophilic cytoplasm, C cells, large with non‐basophilic cytoplasm, and D cells, large with basophilic cytoplasm. The T s of splenic lymphocytes, in four samples not containing Reed‐Sternberg cells, in hours, was: B 11.3; C , 7.9; D , 8.4; combined, 8.8. the T s of B lymphocytes was significantly longer than that of C and D lymphocytes. A lymphocytes did not label sufficiently to measure T s . C and D lymph node lymphocytes and lymphocytes in tissues containing Reed‐Sternberg cells had a longer T s than splenic C and D cells. the former was: B , 12.7; C , 11.7; D , 11.0; combined, 11.8. the latter was: B , 12.2; C , 11.3; D , 11.2; combined, 11.6. the T s of Reed‐Sternberg cells in one specimen of a splenic Hodgkin's tumor was 13.1 hr. Macrophage T s was 10.7 and 15.1 hr. Lymphosarcoma cell T s was 14.2 and 14.6 hr. Reticulum cell sarcoma cell T s was 7.5 and 7.7 hr. The following minimum times were calculated from observation of 3 H‐TdR only labeled mitotic figures: S to prophase, 71 min; S to metaphase, 75 min; S to telophase, 100 min.