Open AccessCHANGES IN THE DNA SYNTHETIC PERIOD DURING THE PHASE OF RAPID GROWTH IN CULTURED DIPLOID FIBROBLASTIC CELLS
Author(s) -
Moore Ruth C.
Publication year - 1971
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1971.tb01556.x
Subject(s) - thymidine , ploidy , dna synthesis , dna , period (music) , tritium , in vitro , biology , mitosis , microbiology and biotechnology , wi 38 , genetics , gene , physics , acoustics , nuclear physics
The DNA synthetic period in diploid fibroblastic cell populations was studied during the phase of rapid growth in vitro , using the labelled mitotic wave method and quantitative autoradiography. It was found that the length of the DNA synthetic period increased progressively, from early passage levels, with the number of generations in vitro. the increase was mainly in the time taken to synthesize the first 50%, and especially the first 10%, of DNA. The change was detectable despite the complication due to a low level of tritium incorporation after the removal of tritiated thymidine from the cultures.