Open AccessDNA SYNTHESIS TIME IN LEUKAEMIC CELLS AS MEASURED BY THE DOUBLE LABELLING AND THE PERCENTAGE LABELLED MITOSES METHODS
Author(s) -
Harriss Eileen B.,
Hoelzer D.
Publication year - 1971
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1971.tb01552.x
Subject(s) - labelling , mitosis , thymidine , in vitro , dna synthesis , peripheral blood , in vivo , cell division , precursor cell , biology , microbiology and biotechnology , cell , andrology , immunology , biochemistry , medicine , genetics
Values of T s provided by the double labelling method have been compared with those given by the percentage labelled mitoses curve for blast cells in the peripheral blood of a patient with plasma cell leukaemia and of rats bearing a transferable acute leukaemia. the double labelling method was carried out giving the first label ( 3 H‐thymidine) in vivo and the second label ( 14 C‐thymidine) in vitro with several values for the interval between the two labels. T s was calculated by fitting regression lines to the results obtained. Data for percentage labelled mitoses were analysed by computer. For the plasma cell leukaemia values of T s = 17.1 ± 7.0 hr and T s = 19.8 ± 3.4 hr, and for the rat leukaemia values of 8.7 ± 1.7 hr and 9.0 ± 1.7 hr (7.1 hr corrected for exponential growth) were obtained from the percentage labelled mitoses and double labelling methods respectively. It is concluded that the double labelling method is valid for the study of cell proliferation in leukaemic blast cells.