Open AccessPERIODICITY OF DNA SYNTHETIC ENZYMES DURING THE HeLa CELL CYCLE
Author(s) -
Brent T. P.
Publication year - 1971
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1971.tb01541.x
Subject(s) - hela , thymidine , enzyme , dna synthesis , thymidine kinase , biochemistry , dna , purine , nucleotide salvage , biosynthesis , deoxycytidine , cell cycle , biology , cell , chemistry , microbiology and biotechnology , nucleotide , genetics , gene , cancer , virus , herpes simplex virus , gemcitabine
The levels of the deoxynucleoside phosphokinases have been determined during the cell cycle in synchronous cultures of HeLa cells. Deoxycytidine and thymidine phosphokinases showed temporally similar periodic fluctuations while the purine deoxynucleoside phosphokinases showed a constant high activity. Experiments with metabolic inhibitors suggest that the observed rise in thymidine phosphokinase activity is directly due to the enzyme's biosynthesis.