Open AccessTHE RELATIONSHIP BETWEEN SPLEEN COLONY PRODUCTION AND SPLEEN CELLULARITY
Author(s) -
Lord B. I.
Publication year - 1971
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1971.tb01531.x
Subject(s) - spleen , biology , andrology , chemistry , microbiology and biotechnology , immunology , medicine
The fraction, f, of injected spleen colony‐forming cells which can be recovered from the spleen of a radiated mouse has been determined at various times up to 24 hr following the initial cell injection. the cellularity of the spleen at the time of assay was also measured and compared with the calculated f number. the linear relationship between these two parameters indicated that over the period of 2‐24 hr the number of CFU/10 6 spleen cells was constant, both falling in a parallel fashion. A further experiment using non‐irradiated W/W v mice in which the spleen size did not change showed the same f number at the end of this period as at 2 hr. It is concluded that CFU are expelled from the spleen during its post‐irradiation contraction thus leading to the apparent fall in f number. It is also concluded that a more realistic f number is obtained by assaying the splenic CFU content 24 hr after the primary cell transfer rather than 2 hr.