Open AccessTHE KINETICS OF GRANULOSA CELLS IN DEVELOPING FOLLICLES IN THE MOUSE OVARY
Author(s) -
Pedersen Torben,
Hartmann Niels Rudolph
Publication year - 1971
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1971.tb01527.x
Subject(s) - mitosis , compartment (ship) , biology , kinetics , cell cycle , microbiology and biotechnology , dna synthesis , thymidine , ovary , andrology , doubling time , dna , cell , endocrinology , biochemistry , physics , medicine , oceanography , quantum mechanics , geology
This investigation describes the kinetics of the granulosa cells in medium‐sized follicles type 3b, 4 and 5a in ovaries of 28‐day‐old Bagg mice. the method of labelling with 3 H‐thymidine followed by high resolution autoradiography is used in the experimental work, which consist of determining percentage labelled mitosis (PLM‐) and continuous labelling (CL‐) curves. In order to analyse the data by computer two alternative hypotheses A and B are set up. Both include the assumptions of no cell loss, exponential growth and a resting compartment Q. In hypothesis A cells from Q re‐enter the mitotic cycle via the normal DNA‐synthesis compartment S p. Hypothesis B includes beside compartment S p a special DNA‐synthesis compartment S q where only cells from Q are synthesizing DNA, and these cells re‐enter the mitotic cycle via the G 2 compartment. the mean transit time in S q is considered to be longer than the mean transit time in S q . On the basis of the hypothesis mathematical expressions for the PLM‐ and CL‐curves are obtained, and by means of a computer the theoretical curves are fitted to the experimental values: thereby all relevant cell kinetical parameters are estimated. Hypothesis B seems to give the best fit between the theoretical and experimental curves. the estimated parameters are: mean cycle times, μ c = (56.1 hr, 56.1 hr and 22.3 hr for type 3b, 4 and 5a respectively), doubling times, T D = (96.4 hr, 118.6 hr and 59.1 hr) and the proportion of cells in Q, p Q = (0.60, 0.71 and 0.69).