Open AccessTHE EFFECTS OF LOW CONCENTRATIONS OF ACTINOMYCIN D ON THE PROGRESS OF CELLS THROUGH THE CELL CYCLE
Author(s) -
Rickinson A. B.
Publication year - 1970
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1970.tb00341.x
Subject(s) - mitosis , cell cycle , dna synthesis , microbiology and biotechnology , cell , dna , cell growth , biology , dactinomycin , cell culture , rna , chemistry , biochemistry , genetics , gene
The progress of L‐cells through the cell cycle in asynchronous and in synchronous culture has been studied at a concentration of actinomycin D which mediates an apparently‘nucleolar‐specific’inhibition of RNA synthesis. Under such conditions, cells may be blocked or seriously delayed in the G 1 and G 2 phases of the cycle whilst the processes of DNA synthesis and mitosis once initiated, can still occur at control rates. The results show that the sensitivity of a cell to these blocks depends critically upon the position of that cell within the cycle at the time of drug addition. The possible mechanisms of the drug's action are discussed.