Open AccessTHE USE OF 59 Fe UPTAKE FOR THE EVALUATION OF CELL PROLIFERATION IN SPLEEN COLONIES
Author(s) -
Siracusa G.,
Silini G.,
Bricanti G.
Publication year - 1970
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1970.tb00272.x
Subject(s) - spleen , population , bone marrow , cell , biology , kinetics , nodule (geology) , microbiology and biotechnology , immunology , chemistry , biochemistry , medicine , physics , paleontology , environmental health , quantum mechanics
A technique of 59 Fe uptake in the spleen of irradiated bone‐marrow‐transplanted mice has been applied to the study of some kinetic parameters of the erythroid spleen nodule cells. Measurements of the doubling time of this population have been obtained together with estimates of radioactivity disappearance from the spleen, owing to a release of nodule cells into the blood stream. The kinetics of expansion of the erythroid cell compartment is compared with the pattern of increase of the colony‐forming cells in the spleen. The expansion of the erythroid cell population seems to be the result of at least two processes: an increase in the number of differentiating cells and the release of mature cells into the blood stream. It is suggested that more refined attempts to evaluate kinetic parameters of the spleen nodule cell population should allow for these factors.