Open AccessFRACTIONATION OF SUSPENSIONS OF MOUSE SPLEEN CELLS BY COUNTER CURRENT DISTRIBUTION
Author(s) -
Brunette D. M.,
McCulloch E. A.,
Till J. E.
Publication year - 1968
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1968.tb00961.x
Subject(s) - spleen , polyethylene glycol , dextran , haematopoiesis , distribution (mathematics) , fractionation , chromatography , current (fluid) , chemistry , biology , biophysics , immunology , microbiology and biotechnology , biochemistry , stem cell , mathematical analysis , mathematics , engineering , electrical engineering
Suspensions of mouse spleen cells were fractionated by means of counter current distribution in an aqueous two‐phase polymer system composed of 5% (w/w) dextran and 4% (w/w) polyethylene glycol. Counter current distribution in this combination of polymers was not toxic for hemopoietic colony‐forming units (CFU) and yielded a partial separation of CFU, granulocytes and antibody‐producing cells. The results obtained indicate that counter current distribution is a useful means for the separation of populations of intact, viable hemopoietic cells.