Open AccessCLONE SIZE ANALYSIS: A PARAMETER IN THE STUDY OF CELL POPULATION KINETICS
Author(s) -
Nias A. H. W.
Publication year - 1968
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1968.tb00956.x
Subject(s) - clone (java method) , irradiation , population , cell size , biology , cell , genetics , microbiology and biotechnology , physics , medicine , nuclear physics , gene , environmental health
The method of clone size analysis is described. Such an analysis provides a measure of the distribution of generation times in a cell population. Treatment by ionizing irradiation leads to perturbation of the generation times of a cell population and such perturbations are shown by changes in the clone size distribution. Distributions were compared after neutron irradiation and β‐irradiation with those after X‐rays; and also between X‐irradiation under aerobic and anoxic conditions. At the same level of cell survival no differences were found between the patterns of cell survival with respect to generation times. Other uses of clone size analysis are discussed.