Open AccessTHE EFFECTS OF CYTOSINE ARABINOSIDE ON THE CELL CYCLE OF CULTURED HUMAN LEUCOCYTES: A MICRODENSITOMETRIC AND AUTORADIOGRAPHIC STUDY
Author(s) -
Brehaut Loreen A.,
Fitzgerald P. H.
Publication year - 1968
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1968.tb00955.x
Subject(s) - cytosine , feulgen stain , dna synthesis , cell cycle , biology , mitosis , dna , thymidine , mitotic index , chromosome , microbiology and biotechnology , cell , biochemistry , gene
PHA‐stimulated leucocytes treated with cytosine arabinoside showed a changed uptake of [ 3 H]thymidine, a lowered mitotic index and chromosome damage. Combined autoradiography and Feulgen microdensitometry demonstrated inhibition of DNA synthesis. Cells in the S period of the cell cycle were arrested in their progress, and cells newly entering S accumulated at the beginning of this period. At stronger concentrations these effects on the cell cycle were complicated by the cytotoxic effect of cytosine arabinoside. The inhibition of DNA synthesis is considered in the light of the chromosomal damage and megaloblastic changes caused by cytosine arabinoside, and is compared to the different pattern of DNA arrest found in megaloblastic anaemia.