Open AccessASSESSMENT OF VIABILITY OF FROZEN BONE MARROW CELLS USING A CELL‐CULTURE METHOD
Author(s) -
Abrahams S.,
Till J. E.,
McCulloch E. A.,
Siminovitch L.
Publication year - 1968
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1968.tb00322.x
Subject(s) - bone marrow , spleen , viability assay , cell , cell culture , biology , immunology , microbiology and biotechnology , biochemistry , genetics
The survival of mouse bone marrow after freezing and thawing was assessed using two methods: the spleen‐colony technique; and a cell‐culture method. The results of these experiments indicate that both methods yield similar estimates of marrow cell viability. It is suggested that the cell‐culture method be used as an assay of marrow‐cell viability in situations where the spleen‐colony technique is not applicable.