Open AccessDISTURBANCES IN THE DEVELOPMENT OF SMALL COLONIES FOLLOWING THE IRRADIATION OF DIFFERENT PARTS OF SINGLE CELLS IN TISSUE CULTURE WITH A HETEROCHROMATIC MICROBEAM OF ULTRAVIOLET LIGHT
Author(s) -
Smith C. L.,
Dendy P. P.
Publication year - 1968
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1968.tb00320.x
Subject(s) - irradiation , microbeam , cytoplasm , biology , incubation , heterochromatin , nucleus , ultraviolet , ultraviolet light , ultraviolet radiation , biophysics , dna , microbiology and biotechnology , chemistry , radiochemistry , optics , biochemistry , nuclear medicine , medicine , physics , photochemistry , nuclear physics , chromatin
Various sites in mouse L cells have been irradiated using an heterochromatic ultraviolet microbeam 3.5 μ in diameter, and the subsequent growth of the cells has been studied for 5–7 days after irradiation. Modifications in the growth curves were recorded for the total number of cells in each radiation category and significant anomalies in the DNA metabolism of some of the progeny of irradiated cells were noted at the end of the post‐irradiaton incubation period. The frequency with which binucleate and giant cells were produced was also recorded and the results analysed. For equivalent absorbed doses, the nucleus is more sensitive than the cytoplasm in experiments of this type and there was some evidence to suggest that the maximum sensitivity is achieved when some nucleolar material is irradiated.