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Macrophages and mesenchymal stromal cells support survival and proliferation of multiple myeloma cells
Author(s) -
Kim Jaehyup,
Denu Ryan A.,
Dollar Bridget A.,
Escalante Leah E.,
Kuether Justin P.,
Callander Natalie S.,
Asimakopoulos Fotis,
Hematti Peiman
Publication year - 2012
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2012.09154.x
Subject(s) - mesenchymal stem cell , stromal cell , bone marrow , plasma cell , cell culture , tumor microenvironment , macrophage , cancer research , cell growth , microbiology and biotechnology , biology , in vitro , chemistry , immunology , tumor cells , biochemistry , genetics
Summary Multiple myeloma (MM) is characterized by almost exclusive tropism of malignant cells for the bone marrow (BM) milieu. The survival and proliferation of malignant plasma cells have been shown to rely on interactions with nonmalignant stromal cells, in particular mesenchymal stromal cells (MSCs), in the BM microenvironment. However, the BM microenvironment is composed of a diverse array of cell types. This study examined the role of macrophages, an abundant component of BM stroma, as a potential niche component that supports malignant plasma cells. We investigated the proliferation of MM tumour cell lines when cultured alone or together with MSCs, macrophages, or a combination of MSCs and macrophages, using the carboxyfluorescein succinimidyl ester assay. Consistently, we observed increased proliferation of MM cell lines in the presence of either MSCs or macrophages compared to cell line‐only control. Furthermore, the combined co‐culture of MSCs plus macrophages induced the greatest degree of proliferation of myeloma cells. In addition to increased proliferation, MSCs and macrophages decreased the rate of apoptosis of myeloma cells. Our in vitro studies provide evidence that highlights the role of macrophages as a key component of the BM microenvironment facilitating the growth of malignant plasma cells in MM.